Human MIF Recombinant Protein (RPPB0759)
- SKU:
- RPPB0759
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P14174
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Human MIF Recombinant Protein |
| Product Code: | RPPB0759 |
| Size: | 25µg |
| Species: | Human |
| Target: | MIF |
| Synonyms: | Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | 1mg/ml solution containing 50mM Tris-HCl pH-8, 0.5mM DTT & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI� SPDRVYINYY DMNAANVGWN NSTFA |
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
MIF human Recombinant was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 12 kDa.
| UniProt Protein Function: | MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase. |
| UniProt Protein Details: | Protein type:EC 5.3.2.1; Isomerase; EC 5.3.3.12; Amino Acid Metabolism - tyrosine; Cell development/differentiation; Cytokine; Apoptosis; Amino Acid Metabolism - phenylalanine Chromosomal Location of Human Ortholog: 22q11.23 Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; vesicle Molecular Function:chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein binding; receptor binding Biological Process: blood coagulation; carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; inflammatory response; innate immune response; leukocyte migration; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; prostaglandin biosynthetic process; regulation of macrophage activation Disease: Rheumatoid Arthritis, Systemic Juvenile |
| NCBI Summary: | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14174 |
| NCBI GenInfo Identifier: | 1170955 |
| NCBI Gene ID: | 4282 |
| NCBI Accession: | P14174.4 |
| UniProt Secondary Accession: | P14174,Q2V4Y5, Q6FHV0, A5Z1R8, B2R4S3, |
| UniProt Related Accession: | P14174 |
| Molecular Weight: | 12,476 Da |
| NCBI Full Name: | Macrophage migration inhibitory factor |
| NCBI Synonym Full Names: | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| NCBI Official Symbol: | MIF�� |
| NCBI Official Synonym Symbols: | GIF; GLIF; MMIF�� |
| NCBI Protein Information: | macrophage migration inhibitory factor |
| UniProt Protein Name: | Macrophage migration inhibitory factor |
| UniProt Synonym Protein Names: | Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase |
| Protein Family: | MIF-like protein |
| UniProt Gene Name: | MIF�� |
| UniProt Entry Name: | MIF_HUMAN |
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