Human MIF (Macrophage Migration Inhibitory Factor) CLIA Kit
The Human MIF (Macrophage Migration Inhibitory Factor) CLIA Kit is designed for the accurate detection of MIF levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.MIF is a key cytokine involved in regulating the immune response and inflammation. It plays a crucial role in various diseases, including autoimmune disorders, inflammatory conditions, and cancer.
By accurately measuring MIF levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially identify new therapeutic targets.With its user-friendly protocols and high-quality components, the Human MIF CLIA Kit from AssayGenie is a valuable tool for researchers looking to study the role of MIF in health and disease. Trust AssayGenie for accurate and reliable results in your research endeavors.
Product Name:
Human MIF (Macrophage Migration Inhibitory Factor) CLIA Kit
SKU:
HUES00867
Target:
Human MIF (Macrophage Migration Inhibitory Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MIF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MIF. You can calculate the concentration of Human MIF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
94-107
85-96
Average (%)
106
100
90
1:4
Range (%)
90-106
92-109
98-111
Average (%)
98
100
105
1:8
Range (%)
87-99
89-101
97-115
Average (%)
94
96
105
1:16
Range (%)
98-112
90-104
97-112
Average (%)
103
97
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
93-110
100
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.28
3.73
0.52
1.17
3.45
Standard deviation
0.05
0.1
0.35
0.06
0.14
0.24
C V (%)
9.43
7.81
9.38
11.54
11.97
6.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MIF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIF in samples. No significant cross-reactivity or interference between Human MIF and analogues was observed.
