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Human Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b) ELISA Kit

SKU:
HUDL01787
Product Type:
ELISA Kit
Sensitivity:
0.068ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MAP1A/1BLC3,ATG8F,Autophagy-related ubiquitin-like modifier LC3 B,MAP1 light chain 3-like protein 2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$899
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Description

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Human Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b) ELISA Kit

The Human Microtubule-Associated Protein 1 Light Chain 3 Beta (MAP1LC3B) ELISA Kit is a powerful tool for accurately measuring levels of MAP1LC3B in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers trustworthy and consistent results, perfect for a wide array of research endeavors.MAP1LC3B is a key player in autophagy, a crucial cellular process involved in maintaining cellular homeostasis and combating various diseases.

Its dysregulation has been implicated in a range of conditions, including cancer, neurodegenerative disorders, and infectious diseases, underscoring its significance as a biomarker for understanding disease mechanisms and exploring potential therapeutic interventions.Invest in the Human MAP1LC3B ELISA Kit to unlock new insights into autophagy and its implications in health and disease.

Product Name:Human Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b) ELISA Kit
Product Code:HUDL01787
Size:96 Assays
Target:MAP1LC3B
Synonyms:MAP1A/1BLC3, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9995
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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