Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) ELISA Kit
The Human MHCC (HLA-C) ELISA Kit is specifically designed for the precise measurement of Major Histocompatibility Complex Class I-C levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for various research purposes.Major Histocompatibility Complex Class I-C is a key component in immune response regulation, playing a crucial role in antigen presentation and immune system function.
Dysregulation of HLA-C expression is associated with various autoimmune diseases, infections, and transplant rejection, highlighting its importance as a target for therapeutic interventions.With the Human MHCC (HLA-C) ELISA Kit, researchers can effectively study the role of HLA-C in immune-related disorders and explore potential treatment options. Its reliable performance and ease of use make it a valuable tool for advancing understanding in immunology and related fields.
Product Name:
Human MHCC/HLA-C (Major Histocompatibility Complex Class I C) ELISA Kit
SKU:
HUES03206
Target:
Human MHCC/HLA-C (Major Histocompatibility Complex Class I C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MHCC/HLA-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MHCC/HLA-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MHCC/HLA-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MHCC/HLA-C. You can calculate the concentration of Human MHCC/HLA-C in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
92-105
97-110
Average (%)
100
97
102
1:4
Range (%)
86-100
80-93
88-100
Average (%)
92
86
93
1:8
Range (%)
91-105
86-97
83-96
Average (%)
98
92
90
1:16
Range (%)
85-98
83-93
86-101
Average (%)
92
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.22
8.18
0.94
2.44
7.67
Standard deviation
0.06
0.1
0.35
0.05
0.1
0.38
C V (%)
6.06
4.5
4.28
5.32
4.1
4.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MHCC/HLA-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MHCC/HLA-C in samples. No significant cross-reactivity or interference between Human MHCC/HLA-C and analogues was observed.
