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Human Lipase, Monoacylglycerol (MGL) ELISA Kit

SKU:
HUDL01841
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q99685
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
HU-K5, MGLL,MAGL,Monoglyceride Lipase,Lysophospholipase homolog,Lysophospholipase-like,Monoacylglycerol lipase
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Lipase, Monoacylglycerol (MGL) ELISA Kit

The Human Lipase Monoacylglycerol (MGL) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of MGL in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for reliable and reproducible results that can be used in a variety of research applications.MGL is an enzyme involved in the breakdown of monoacylglycerols, which are important molecules in lipid metabolism.

Dysregulation of MGL activity has been implicated in various diseases such as obesity, diabetes, and metabolic disorders. By measuring MGL levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.Overall, the Human Lipase Monoacylglycerol (MGL) ELISA Kit is a valuable tool for researchers studying lipid metabolism and related diseases, providing accurate and precise measurements of MGL levels in human samples.

Product Name:Human Lipase, Monoacylglycerol (MGL) ELISA Kit
Product Code:HUDL01841
Size:96 Assays
Target:MGL
Synonyms:HU-K5, MGLL, MAGL, Monoglyceride Lipase, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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