Human Methylthioadenosine Phosphorylase (MTAP) ELISA Kit
The Human Methylthioadenosine Phosphorylase (MTAP) ELISA Kit is a powerful tool for the precise measurement of MTAP levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for various research purposes. MTAP is a key enzyme involved in the methionine salvage pathway, playing a crucial role in maintaining cellular methionine levels and supporting various metabolic processes. Dysregulation of MTAP has been linked to cancer development and progression, highlighting its significance as a potential biomarker for cancer research and therapeutic interventions.
By utilizing the Human MTAP ELISA Kit, researchers can uncover valuable insights into MTAP expression levels, furthering our understanding of its role in health and disease. With its reliability and precision, this kit offers a comprehensive solution for exploring the biological implications of MTAP and advancing scientific discoveries in the field.
Product Name:
Human Methylthioadenosine Phosphorylase (MTAP) ELISA Kit
Product Code:
HUDL01919
Size:
96 Assays
Target:
MTAP
Synonyms:
MSAP, S-methyl-5'-thioadenosine phosphorylase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
