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Human Methyl CpG Binding Protein 2 (MECP2) ELISA Kit

SKU:
HUDL01827
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P51608
Sensitivity:
0.065ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
RTS,AUTSX3,MRX16,MRX79,PPMX,RTT,Rett Syndrome,Mental Retardation,X-linked 16
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Methyl CpG Binding Protein 2 (MECP2) ELISA Kit

The Human Methyl-CpG Binding Protein 2 (MeCP2) ELISA Kit is specifically designed for the quantitative detection of MeCP2 protein levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and accurate results for a variety of research applications.MeCP2 is a key protein involved in regulating gene expression by binding to methylated DNA sequences.

Dysregulation of MeCP2 has been linked to neurological disorders such as Rett syndrome, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its reliable performance and ease of use, the Human MeCP2 ELISA Kit is an essential tool for researchers investigating the role of MeCP2 in disease pathogenesis and developing targeted treatments.

Product Name:Human Methyl CpG Binding Protein 2 (MECP2) ELISA Kit
Product Code:HUDL01827
Size:96 Assays
Target:MECP2
Synonyms:RTS, AUTSX3, MRX16, MRX79, PPMX, RTT, Rett Syndrome, Mental Retardation,X-linked 16
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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