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Human Metallothionein 3 ELISA Kit

SKU:
HUFI02643
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P25713
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
MT3, Metallothionein 3
Reactivity:
Human
€599
Frequently bought together:

Description

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Human Metallothionein 3 ELISA Kit

The Human Metallothionein-3 ELISA Kit is a powerful tool for measuring metallothionein-3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, delivering consistent and dependable results for various research purposes.Metallothionein-3 is a key protein that plays a vital role in metal homeostasis and detoxification processes within the body. It is known to be involved in various physiological and pathological conditions, including heavy metal toxicity, oxidative stress, and neurodegenerative diseases.

Therefore, studying metallothionein-3 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.With its advanced technology and reliable performance, the Human Metallothionein-3 ELISA Kit is an essential tool for researchers looking to investigate the role of metallothionein-3 in disease pathogenesis and explore its potential as a therapeutic target.

Product Name:Human Metallothionein 3 ELISA Kit
Product Code:HUFI02643
Size:96 Assays
Alias:MT3, Metallothionein 3
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human MT3 concentrations in plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human MT3 and the recovery rates were calculated by comparing the measured value to the expected amount of Human MT3 in samples.
MatrixRecovery range(%)Average(%)
EDTA plasma(n=5)87-10598
UFH plasma(n=5)89-10092
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human MT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
EDTA plasma(n=5)87-96%82-101%91-100%
UFH plasma(n=5)85-100%84-95%82-97%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP25713
UniProt Protein Function:MT3: Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro. Belongs to the metallothionein superfamily. Type 1 family.
UniProt Protein Details:Chromosomal Location of Human Ortholog:16q13

Cellular Component:synaptic vesicle; perinuclear region of cytoplasm; cytoplasm; inclusion body; intracellular

Molecular Function:antioxidant activity; protein binding; copper ion binding; zinc ion binding; caspase inhibitor activity; drug binding; cadmium ion binding; protein kinase activator activity

Biological Process:positive regulation of catalytic activity; negative regulation of autophagy; removal of superoxide radicals; positive regulation of transcription, DNA-dependent; negative regulation of axon extension; negative regulation of caspase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; zinc ion homeostasis; cadmium ion homeostasis; cellular lipid catabolic process; histone modification; protein kinase B signaling cascade; negative regulation of neuron apoptosis; cholesterol catabolic process; regulation of response to food; protein stabilization; cellular zinc ion homeostasis; activation of protein kinase B; zinc ion transport; regulation of protein amino acid glycosylation; astrocyte development; leptin-mediated signaling pathway; cell proliferation; cellular metal ion homeostasis; protein import into nucleus, translocation; negative regulation of oxidoreductase activity; response to hypoxia; energy reserve metabolic process; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

UniProt Code:P25713
NCBI GenInfo Identifier:5174762
NCBI Gene ID:4504
NCBI Accession:NP_005945.1
UniProt Secondary Accession:P25713,Q2V574,
UniProt Related Accession:P25713
Molecular Weight:6,927 Da
NCBI Full Name:metallothionein-3
NCBI Synonym Full Names:metallothionein 3
NCBI Official Symbol:MT3Â Â
NCBI Official Synonym Symbols:GIF; GIFB; GRIF; ZnMT3Â Â
NCBI Protein Information:metallothionein-3; MT-3; MT-III; metallothionein-III; growth inhibitory factor; metallothionein 3 (growth inhibitory factor (neurotrophic))
UniProt Protein Name:Metallothionein-3
UniProt Synonym Protein Names:GIFB; GIF; Growth inhibitory factor; Metallothionein-III
Protein Family:Metallothionein
UniProt Gene Name:MT3Â Â
UniProt Entry Name:MT3_HUMAN

*Note:Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14.Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.Note:Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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