The Human Metallothionein-2 (MT2) ELISA Kit is a reliable and accurate assay designed for the detection of metallothionein-2 levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures consistent and reproducible results, making it suitable for various research applications.Metallothionein-2 is a vital protein known for its role in metal detoxification and regulation of essential metal ions in the body. It is involved in various physiological processes, including antioxidant defense and metal homeostasis.
Dysregulation of metallothionein-2 has been implicated in diseases such as cancer, neurodegenerative disorders, and metal poisoning, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.Overall, the Human Metallothionein-2 (MT2) ELISA Kit is a valuable tool for researchers interested in investigating the role of metallothionein-2 in health and disease, providing accurate and reliable quantification of this essential protein in biological samples.
Product Name:
Human Metallothionein 2 (MT2) ELISA Kit
Product Code:
HUDL01917
Size:
96 Assays
Target:
MT2
Synonyms:
MT2A, CES1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
