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Human Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) ELISA Kit

SKU:
HUDL01906
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11836
Sensitivity:
0.45ng/mL
Range:
1.25-80ng/mL
ELISA Type:
Sandwich
Synonyms:
CD20,MS4-A1,B1,Bp35,LEU16,MS4-A2,S7,B-lymphocyte antigen CD20,B-lymphocyte surface antigen B1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) ELISA Kit

The Human MS4A1 (Membrane-Spanning 4-Domains Subfamily A Member 1) ELISA Kit is specifically designed for the accurate and sensitive detection of MS4A1 levels in human serum, plasma, and cell culture supernatants. This kit is highly reliable and provides reproducible results, making it a valuable tool for various research applications.MS4A1 is a protein that belongs to the MS4A membrane protein family and is involved in immune responses and cell signaling.

It is known to play a role in various diseases, including cancer, autoimmune disorders, and neuroinflammatory conditions. Therefore, measuring MS4A1 levels can provide important insights into the pathogenesis of these diseases and help in the development of targeted therapies.With its high sensitivity and specificity, the Human MS4A1 ELISA Kit is a must-have for researchers studying MS4A1 biology and its implications in human health and disease.

Product Name:Human Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) ELISA Kit
Product Code:HUDL01906
Size:96 Assays
Target:MS4A1
Synonyms:CD20, MS4-A1, B1, Bp35, LEU16, MS4-A2, S7, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1
Detection Method:Sandwich
Range:1.25-80ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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