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Human Melanoma Inhibitory Activity Protein 1 (MIA1) ELISA Kit

SKU:
HUDL01851
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q16674
Sensitivity:
25pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD-RAP,Melanoma-derived growth regulatory protein
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Human Melanoma Inhibitory Activity Protein 1 (MIA1) ELISA Kit

The Human Melanoma Inhibitory Activity Protein 1 (MIA1) ELISA Kit is specifically designed for the precise detection of MIA1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an ideal choice for a variety of research purposes. MIA1 is a crucial protein involved in melanoma development and progression, playing a key role in inhibiting melanoma cell growth and metastasis.

By measuring MIA1 levels, researchers can better understand the mechanisms underlying melanoma and develop potential treatments for this aggressive form of skin cancer. With its high-quality components and user-friendly protocol, the Human MIA1 ELISA Kit from Assay Genie provides researchers with a valuable tool for studying melanoma biology and identifying new therapeutic targets.

Product Name:Human Melanoma Inhibitory Activity Protein 1 (MIA1) ELISA Kit
Product Code:HUDL01851
Size:96 Assays
Target:MIA1
Synonyms:CD-RAP, Melanoma-derived growth regulatory protein
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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