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Human Melanoma Derived Leucine Zipper Extra Nuclear Factor (MLZE) ELISA Kit

SKU:
HUDL01867
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9BYG8
Sensitivity:
0.066ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GSDMC,Gasdermin C,Melanoma-Derived Leucine Zipper,Extranuclear Factor
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Melanoma Derived Leucine Zipper Extra Nuclear Factor (MLZE) ELISA Kit

The Human Melanoma-Derived Leucine Zipper Extra Nuclear Factor (MLZE) ELISA Kit is specifically designed for the precise detection of MLZE levels in human cell lysates, tissue homogenates, and biological fluids. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for a variety of research applications.MLZE is a key regulatory protein involved in melanoma development and progression, playing a crucial role in cell proliferation, invasion, and metastasis.

By accurately measuring MLZE levels, researchers can gain valuable insights into the molecular mechanisms underlying melanoma and identify potential therapeutic targets for this aggressive form of skin cancer.Overall, the Human Melanoma-Derived Leucine Zipper Extra Nuclear Factor (MLZE) ELISA Kit is an invaluable tool for investigating the role of MLZE in melanoma pathogenesis and advancing our understanding of this complex disease.

Product Name:Human Melanoma Derived Leucine Zipper Extra Nuclear Factor (MLZE) ELISA Kit
Product Code:HUDL01867
Size:96 Assays
Target:MLZE
Synonyms:GSDMC, Gasdermin C, Melanoma-Derived Leucine Zipper,Extranuclear Factor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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