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Human Melanoma Cell Adhesion Molecule (MCAM) ELISA Kit

SKU:
HUDL01809
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P43121
Sensitivity:
1.21ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
CD146,MUC18,Cell surface glycoprotein P1H12,Melanoma-associated antigen A32,S-endo 1 endothelial-associated antigen
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Melanoma Cell Adhesion Molecule (MCAM) ELISA Kit

The Human Melanoma Cell Adhesion Molecule (MCAM) ELISA Kit is a powerful tool for the precise measurement of MCAM levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it a valuable asset for various research endeavors. MCAM, also known as CD146, is a key molecule involved in cell adhesion and migration, playing a critical role in processes like tumor progression and metastasis.

By detecting MCAM levels, researchers can gain insights into the mechanisms underlying melanoma development and potentially identify new therapeutic targets for this aggressive skin cancer. This ELISA kit offers a comprehensive solution for studying MCAM in melanoma and other related conditions, providing researchers with the tools they need to advance their understanding of disease progression and potentially develop innovative treatment strategies.

Product Name:Human Melanoma Cell Adhesion Molecule (MCAM) ELISA Kit
Product Code:HUDL01809
Size:96 Assays
Target:MCAM
Synonyms:CD146, MUC18, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, S-endo 1 endothelial-associated antigen
Detection Method:Sandwich
Range:3.125-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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