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Human MED21 Recombinant Protein (RPPB3953)

SKU:
RPPB3953
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13503
€309
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Description

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Product Name:Human MED21 Recombinant Protein
Product Code:RPPB3953
Size:10µg
Species:Human
Target:MED21
Synonyms:SRB7, SURB7, Mediator complex subunit 21, RNA polymerase II holoenzyme component SRB7, hSrb7.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:MED21 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMADRLTQ LQDAVNSLAD QFCNAIGVLQ QCGPPASFNN IQTAINKDQP ANPTEEYAQL FAALIARTAK DIDVLIDSLP SEESTAALQA ASLYKLEEEN HEAATCLEDV VYRGDMLLEK IQSALADIAQ SQLKTRSGTH SQSLPDS

Mediator Complex Subunit 21 (MED21) belongs to the mediator complex subunit 21 family. MED21 interacts with the human RNA polymerase II holoenzyme and is participated in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of MED21 can be found on chromosome 8. Alternative splicing results in multiple transcript variants.

MED21 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-144 a.a.) and having a molecular mass of 18.0kDa.MED21 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:MED21: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 21 family.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12p11.23

Cellular Component: Srb-mediator complex

Molecular Function:DNA-directed RNA polymerase activity; protein binding; transcription coactivator activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
UniProt Code:Q13503
NCBI GenInfo Identifier:7531204
NCBI Gene ID:9412
NCBI Accession:Q13503.1
UniProt Secondary Accession:Q13503,Q6IB05, Q92811, B2R4I3,
UniProt Related Accession:Q13503
Molecular Weight:16kDa
NCBI Full Name:Mediator of RNA polymerase II transcription subunit 21
NCBI Synonym Full Names:mediator complex subunit 21
NCBI Official Symbol:MED21��
NCBI Official Synonym Symbols:SRB7; SURB7; hSrb7��
NCBI Protein Information:mediator of RNA polymerase II transcription subunit 21
UniProt Protein Name:Mediator of RNA polymerase II transcription subunit 21
UniProt Synonym Protein Names:Mediator complex subunit 21; RNA polymerase II holoenzyme component SRB7; RNAPII complex component SRB7; hSrb7
Protein Family:Putative mediator of RNA polymerase II transcription
UniProt Gene Name:MED21��

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