Human MeCP2 (Methyl CpG Binding Protein 2) ELISA Kit
The Human MECP2 (Methyl-CpG Binding Protein 2) ELISA Kit is a reliable tool for the precise measurement of MECP2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and accurate results, making it suitable for various research applications.MECP2 is a key protein involved in regulating gene expression through DNA methylation. Dysregulation of MECP2 has been linked to neurodevelopmental disorders such as Rett syndrome, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Human MECP2 ELISA Kit is a valuable asset for researchers looking to investigate the role of MECP2 in health and disease, providing a reliable and efficient method for quantifying MECP2 levels in biological samples.
Product Name:
Human MeCP2 (Methyl CpG Binding Protein 2) ELISA Kit
SKU:
HUES03424
Target:
Human MeCP2 (Methyl CpG Binding Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MeCP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MeCP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MeCP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MeCP2. You can calculate the concentration of Human MeCP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
86-96
95-108
Average (%)
105
91
100
1:4
Range (%)
88-101
85-98
85-98
Average (%)
93
91
90
1:8
Range (%)
88-104
83-95
81-92
Average (%)
95
87
87
1:16
Range (%)
92-107
81-96
87-101
Average (%)
98
88
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.5
4.75
0.56
1.47
4.48
Standard deviation
0.03
0.08
0.17
0.03
0.08
0.17
C V (%)
5.45
5.33
3.58
5.36
5.44
3.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MeCP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MeCP2 in samples. No significant cross-reactivity or interference between Human MeCP2 and analogues was observed.
