The Human ME20m Melanoma-Associated ME20 CLIA Kit is specifically designed for the accurate detection of the ME20 protein in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing reliable and reproducible results for research applications related to melanoma.ME20 is a protein associated with melanoma, a type of skin cancer. Its detection and study can provide valuable insights into the development and progression of melanoma, as well as potential therapeutic targets.
This kit is essential for researchers and clinicians studying melanoma and developing targeted therapies for this aggressive form of cancer.Overall, the Human ME20m Melanoma-Associated ME20 CLIA Kit is a valuable tool for researchers in the field of oncology, offering precise and reliable measurements for the detection of ME20 in human samples.
Product Name:
Human ME20M (Melanoma Associated ME20) CLIA Kit
SKU:
HUES01263
Target:
Human ME20M (Melanoma Associated ME20)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ME20M. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ME20M and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ME20M, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ME20M. You can calculate the concentration of Human ME20M in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
92-107
86-98
Average (%)
109
99
93
1:4
Range (%)
91-108
99-114
90-101
Average (%)
99
107
96
1:8
Range (%)
89-105
96-107
97-109
Average (%)
96
102
103
1:16
Range (%)
98-115
98-115
93-104
Average (%)
105
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
96-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
90.94
234.58
930.21
89.27
233.53
865.47
Standard deviation
10.06
27.8
84.18
10.52
17.0
63.09
C V (%)
11.06
11.85
9.05
11.78
7.28
7.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ME20M concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ME20M in samples. No significant cross-reactivity or interference between Human ME20M and analogues was observed.
