Human ME2 Recombinant Protein (RPPB1933)
- SKU:
- RPPB1933
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P23368
- Research Area:
- Enzymes
Description
| Product Name: | Human ME2 Recombinant Protein |
| Product Code: | RPPB1933 |
| Size: | 25µg |
| Species: | Human |
| Target: | ME2 |
| Synonyms: | Malic enzyme 2 NAD(+)-dependent mitochondrial, NAD-ME, ODS1, Malate Dehydrogenase, NAD-dependent malic enzyme mitochondrial, pyruvic-malic carboxylase, Malic enzyme 2, EC 1.1.1.38, EC 1.1.1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was Lyophilized from a 0.2�m filtered concentrated solution in 20mM Tris, 150mM NaCl, 1mM b-mercaptoethanol, 1mM EDTA, pH8.0. |
| Solubility: | It is recommended to reconstitute the lyophilized ME2 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized ME2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ME2 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by(a) Analysis by HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MLHIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWPESASSPPVITEHHHHHH |
ME2 catalyzes the oxidative decarboxylation of malate to pyruvate, malat + NAD(P)+ = pyruvate + CO2 + NAD(P)H+, and is found both in eukaryotic and prokaryotic cells. Three different isoforms of ME are known to be in mammalian tissues: a strictly cytosolic NADP+-dependent enzyme, an NADP+-dependent mitochondriail isoform, and a mitochondrial isoenzyme that is able to use both NAD+ and NADP+ but is more effective with NAD+. The mammalian isoforms size is about 62-64 kDa. A native size of 240,000 Da proposes a tetrameric structure for the active enzyme. Mitochondrial NAD+-dependent ME 2 activity is seen in tissues that experience many cell divisions, like spleen, thymus, and the basal cells of the small intestinal mucosa. ME2 is also expressed all through the rapid cleavage stages of early Xenopus development. Activity for this isoform is low or nonexistent in brain, muscle, and normal and regenerating liver tissue from rat but was observed in rat adrenal cortex, pigeon and human skeletal muscle, and in heart muscle of some species. In addition, it is expressed in mitochondria of all tumor cells inspected to detain ascites tumors, hepatoma cells, and a variety of other tumors and transformed cell lines.
ME2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 573 amino acids and having a total molecular mass of 64.4kDa.ME2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ME2: a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009] |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - pyruvate; Oxidoreductase; Mitochondrial; EC 1.1.1.38 Chromosomal Location of Human Ortholog: 18q21 Cellular Component: intracellular membrane-bound organelle; mitochondrion Molecular Function:electron carrier activity; malic enzyme activity Disease: Epilepsy, Idiopathic Generalized; Opioid Dependence, Susceptibility To, 1 |
| NCBI Summary: | This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene. [provided by RefSeq, Dec 2009] |
| UniProt Code: | P23368 |
| NCBI GenInfo Identifier: | 126733 |
| NCBI Gene ID: | 4200 |
| NCBI Accession: | P23368.1 |
| UniProt Secondary Accession: | P23368,Q9BWL6, Q9BYG1, Q9H4B2, B2R8J2, |
| UniProt Related Accession: | P23368 |
| Molecular Weight: | 53,586 Da |
| NCBI Full Name: | NAD-dependent malic enzyme, mitochondrial |
| NCBI Synonym Full Names: | malic enzyme 2 |
| NCBI Official Symbol: | ME2�� |
| NCBI Official Synonym Symbols: | ODS1�� |
| NCBI Protein Information: | NAD-dependent malic enzyme, mitochondrial |
| UniProt Protein Name: | NAD-dependent malic enzyme, mitochondrial |
| UniProt Synonym Protein Names: | Malic enzyme 2 |
| UniProt Gene Name: | ME2�� |
| UniProt Entry Name: | MAOM_HUMAN |
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