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Human Mdm2 p53 Binding Protein Homolog (MDM2) ELISA Kit

SKU:
HUDL01824
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q00987
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
HDMX,hdm2,Mouse Double Minute 2,Human Homolog Of p53-Binding Protein,Oncoprotein Mdm2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Mdm2 p53 Binding Protein Homolog (MDM2) ELISA Kit

The Human MDM2 (Mouse Double Minute 2) ELISA Kit is specifically designed for the quantitative detection of MDM2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.MDM2 is a key protein involved in regulating the activity of the tumor suppressor protein p53, playing a crucial role in cell growth, proliferation, and apoptosis.

Dysregulation of the MDM2-p53 pathway has been implicated in various cancers and other diseases, making MDM2 a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human MDM2 ELISA Kit provides researchers with a reliable tool to quantitatively measure MDM2 levels and further understand the role of this protein in health and disease.

Product Name:Human Mdm2 p53 Binding Protein Homolog (MDM2) ELISA Kit
Product Code:HUDL01824
Size:96 Assays
Target:MDM2
Synonyms:HDMX, hdm2, Mouse Double Minute 2,Human Homolog Of p53-Binding Protein, Oncoprotein Mdm2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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