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Human MCPIP / Monocyte Chemoattractant Protein-induced Protein 1 ELISA Kit

SKU:
HUFI02986
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q5D1E8
Sensitivity:
2.344pg/ml
Range:
3.906-250pg/ml
ELISA Type:
Sandwich
Synonyms:
Ribonuclease ZC3H12A, MCP-induced protein 1, Zinc finger CCCH domain-containing protein 12A, MCPIP1
Reactivity:
Human
Research Area:
Cardiovascular
€599
Frequently bought together:

Description

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Human MCPIP/Monocyte Chemoattractant Protein-induced Protein 1 ELISA Kit

The Human MCPIP (Monocyte Chemoattractant Protein-Induced Protein 1) ELISA Kit is a reliable tool for the precise measurement of MCPIP levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable asset for various research endeavors.MCPIP is a key protein involved in immune response and inflammation, playing a crucial role in regulating gene expression and cell signaling pathways.

Dysregulation of MCPIP has been linked to various diseases including autoimmune disorders, inflammatory conditions, and cancer, highlighting its significance as a potential therapeutic target and diagnostic marker.By utilizing the Human MCPIP ELISA Kit, researchers can gain a deeper understanding of MCPIP's role in disease pathogenesis and explore its therapeutic potential, ultimately advancing the field of immunology and drug development.

Product Name:Human MCPIP / Monocyte Chemoattractant Protein-induced Protein 1 ELISA Kit
Product Code:HUFI02986
Size:96 Assays
Alias:Ribonuclease ZC3H12A, MCP-induced protein 1, Zinc finger CCCH domain-containing protein 12A, MCPIP1
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human MCPIP concentrations in serum plasma and other biological fluids.
Sensitivity:2.344pg/ml
Range:3.906-250pg/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human MCPIP and the recovery rates were calculated by comparing the measured value to the expected amount of Human MCPIP in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)86-10296
EDTA plasma(n=5)90-10596
UFH plasma(n=5)85-10093
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human MCPIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)89-105%86-96%89-104%
EDTA plasma(n=5)83-101%88-101%84-100%
UFH plasma(n=5)82-97%80-97%80-97%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ5D1E8
UniProt Protein Function:MCPIP1: Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA. Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19. Belongs to the ZC3H12 family.
UniProt Protein Details:Protein type:Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: cytoplasm; cytoskeleton; nucleoplasm; nucleus; plasma membrane; rough endoplasmic reticulum

Molecular Function:chromatin binding; DNA binding; endoribonuclease activity; exoribonuclease activity; miRNA binding; mRNA 3'-UTR binding; mRNA binding; protein binding; ribonuclease activity; ribosome binding; RNA binding

Biological Process: cellular response to glucose starvation; inhibition of NF-kappaB transcription factor; mRNA catabolic process, endonucleolytic cleavage-dependent decay; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 beta secretion; negative regulation of interleukin-6 production; negative regulation of macrophage activation; negative regulation of NF-kappaB import into nucleus; negative regulation of nitric oxide biosynthetic process; negative regulation of protein amino acid phosphorylation; negative regulation of tumor necrosis factor production; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of defense response to virus by host; positive regulation of fat cell differentiation; positive regulation of protein import into nucleus; positive regulation of transcription from RNA polymerase II promoter; protein deubiquitination; protein oligomerization; regulation of gene expression

NCBI Summary:ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM, Mar 2008]
UniProt Code:Q5D1E8
NCBI GenInfo Identifier:190479827
NCBI Gene ID:80149
NCBI Accession:Q5D1E8.1
UniProt Secondary Accession:Q5D1E8,Q6I9Z1, Q9H5P1, D3DPT0,
UniProt Related Accession:Q5D1E8
Molecular Weight:65,699 Da
NCBI Full Name:Bifunctional endoribonuclease and deubiquitinase ZC3H12A
NCBI Synonym Full Names:zinc finger CCCH-type containing 12A
NCBI Official Symbol:ZC3H12A
NCBI Official Synonym Symbols:MCPIP; MCPIP1; dJ423B22.1
NCBI Protein Information:bifunctional endoribonuclease and deubiquitinase ZC3H12A
UniProt Protein Name:Bifunctional endoribonuclease and deubiquitinase ZC3H12A
UniProt Synonym Protein Names:Monocyte chemotactic protein-induced protein 1
Protein Family:Ribonuclease
UniProt Gene Name:ZC3H12A
UniProt Entry Name:ZC12A_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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