Human MCP/CD46 (Membrane Cofactor Protein) CLIA Kit
The Human MCP-CD46 (Membrane Cofactor Protein) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MCP-CD46 levels in human biological samples. This kit is ideal for research applications in immunology, oncology, and inflammatory diseases.MCP-CD46 is a membrane-bound regulator protein that plays a key role in the immune response and complement system. Dysregulation of MCP-CD46 has been linked to various autoimmune diseases, infections, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing therapeutic strategies.
With its reliable performance and ease of use, the Human MCP-CD46 CLIA Kit offers researchers a valuable tool for investigating the role of MCP-CD46 in health and disease. Order yours today and take your research to the next level.
Product Name:
Human MCP/CD46 (Membrane Cofactor Protein) CLIA Kit
SKU:
HUES00976
Target:
Human MCP/CD46 (Membrane Cofactor Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MCP/CD46. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP/CD46 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP/CD46, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MCP/CD46. You can calculate the concentration of Human MCP/CD46 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-110
101-113
96-106
Average (%)
104
107
101
1:4
Range (%)
83-98
93-105
101-115
Average (%)
90
99
107
1:8
Range (%)
101-115
100-113
97-111
Average (%)
109
107
105
1:16
Range (%)
94-110
93-105
98-115
Average (%)
102
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
94
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.16
39.36
149.32
22.62
41.43
155.0
Standard deviation
2.7
3.25
11.77
2.5
3.3
15.44
C V (%)
12.76
8.26
7.88
11.05
7.97
9.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MCP/CD46 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MCP/CD46 in samples. No significant cross-reactivity or interference between Human MCP/CD46 and analogues was observed.
