Human MCP-2/CCL8 (Monocyte Chemotactic Protein 2) CLIA Kit
The Human MCP-2 (CCL8) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Monocyte Chemotactic Protein 2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Monocyte Chemotactic Protein 2 (MCP-2) is a critical chemokine involved in the recruitment and activation of monocytes during inflammation and immune responses.
Dysregulation of MCP-2 has been implicated in various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.With its high sensitivity and specificity, the Human MCP-2 (CCL8) CLIA Kit from Assay Genie offers researchers a reliable tool for measuring MCP-2 levels in human samples, providing valuable insights into the role of this chemokine in health and disease.
Product Name:
Human MCP-2/CCL8 (Monocyte Chemotactic Protein 2) CLIA Kit
SKU:
HUES00669
Target:
Human MCP-2/CCL8 (Monocyte Chemotactic Protein 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-2/CCL8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-2/CCL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-2/CCL8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MCP-2/CCL8. You can calculate the concentration of Human MCP-2/CCL8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
101-118
102-119
Average (%)
96
108
109
1:4
Range (%)
103-119
95-111
96-108
Average (%)
109
102
102
1:8
Range (%)
88-100
102-118
98-112
Average (%)
93
109
106
1:16
Range (%)
89-104
88-103
88-100
Average (%)
96
95
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
105
EDTA plasma (n=5)
98-110
105
Cell culture media (n=5)
99-117
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.46
114.48
306.04
45.21
106.03
277.17
Standard deviation
4.8
10.36
28.16
4.77
8.2
27.33
C V (%)
11.04
9.05
9.2
10.55
7.73
9.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MCP-2/CCL8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MCP-2/CCL8 in samples. No significant cross-reactivity or interference between Human MCP-2/CCL8 and analogues was observed.
