Human MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
The MCP-1 ELISA Kit is expertly crafted to enable the quantitative detection of Monocyte Chemotactic Protein 1 (MCP-1) levels in human biological samples. MCP-1, a key chemokine involved in inflammatory processes, attracts monocytes and promotes their recruitment to sites of inflammation. Understanding the levels of MCP-1 is vital as it plays a critical role in immune response modulation, cell migration, and progression of inflammatory conditions. Accurate measurement of MCP-1 facilitates insights into its involvement in various diseases, immune disorders, and infections, aiding in the development of targeted treatment strategies.
Our MCP-1 ELISA Kit is designed to deliver outstanding sensitivity and specificity, ensuring precise and reproducible results. Manufactured under rigorous quality standards, this kit ensures robust performance and user-friendly operations, making it an ideal choice for both research and clinical applications. Trust our MCP-1 ELISA Kit for dependable and precise quantification of this essential biomarker in your scientific studies and investigations.
Product Name:
Human MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit
SKU:
AEES00045
Target:
Human MCP-1 (Monocyte Chemotactic Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MCP-1. You can calculate the concentration of Human MCP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
89-102
91-104
Average (%)
103
96
98
1:4
Range (%)
87-98
90-101
94-100
Average (%)
92
94
97
1:8
Range (%)
90-100
94-103
101-111
Average (%)
94
99
105
1:16
Range (%)
89-99
84-96
89-101
Average (%)
96
92
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-108
101
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
87-95
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.91
488.33
1680.55
164.36
433.06
1730.78
Standard deviation
10.01
24.12
90.58
9.45
20.74
76.85
C V (%)
5.03
4.94
5.39
5.75
4.79
4.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MCP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MCP-1 in samples. No significant cross-reactivity or interference between Human MCP-1 and analogues was observed.
