The Human Myelin Basic Protein (MBP) ELISA Kit is meticulously crafted to quantify MBP levels accurately in human biological specimens.
MBP, a key structural protein found in the myelin sheath of nerve cells, plays a critical role in nerve transmission and maintenance of the nervous system. Aberrant MBP levels have been associated with demyelinating disorders such as multiple sclerosis and other neurodegenerative conditions, highlighting the importance of precise MBP measurements in elucidating nervous system function and pathophysiology. This ELISA kit provides exceptional sensitivity and specificity, enabling researchers to investigate MBP levels with high precision. By facilitating the quantitative assessment of MBP, this kit serves as a valuable tool in neurology research, offering insights into myelin structure, neuronal health, and neurodegenerative diseases. The accurate measurement of MBP levels using this kit can aid in understanding the role of myelin in neurological disorders, disease progression, and the development of potential therapeutic strategies. Manufactured under stringent quality assurance measures, the Human MBP ELISA Kit ensures reliable and reproducible performance, making it an ideal choice for researchers seeking to advance their understanding of myelin biology, neurodegenerative disorders, and nervous system function. With its user-friendly design and robust analytical capabilities, this kit empowers researchers to delve deeper into the intricacies of myelin biology and its implications in neurological health and disease.
Product Name:
Human MBP (Myelin Basic Protein) ELISA Kit
SKU:
AEES00675
Target:
Human MBP (Myelin Basic Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MBP. You can calculate the concentration of Human MBP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
89-103
85-100
Average (%)
106
96
91
1:4
Range (%)
95-106
90-102
85-95
Average (%)
101
96
90
1:8
Range (%)
101-114
91-106
84-94
Average (%)
108
97
89
1:16
Range (%)
99-113
86-101
82-93
Average (%)
105
92
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.04
188.54
921.9
107.47
202.06
900.84
Standard deviation
5.99
10.48
32.54
7.19
9.88
37.93
C V (%)
5.6
5.56
3.53
6.69
4.89
4.21
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MBP concentrations in .
Specificity:
This kit recognizes Human MBP in samples. No significant cross-reactivity or interference between Human MBP and analogues was observed.
