The MAU (Microalbuminuria) ELISA Kit is meticulously crafted to facilitate the precise and quantitative detection of Microalbuminuria levels in various human samples. Microalbuminuria, an early marker for kidney dysfunction and cardiovascular risks, plays a pivotal role in identifying renal abnormalities and predicting cardiovascular events. Its detection is essential for the early diagnosis and management of kidney diseases and related complications.
This ELISA kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results. Manufactured with stringent quality controls, it delivers robust performance and user-friendly operation, making it an ideal choice for both research and clinical applications. Trust the reliability of Assay Genie's MAU ELISA Kit for accurate and dependable quantification of this critical biomarker in your studies.
Product Name:
Human MAU (Microalbuminuria) ELISA Kit
SKU:
AEES00054
Target:
Human MAU (Microalbuminuria)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 pg/mL
Detection range:
0.78-50 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MAU. You can calculate the concentration of Human MAU in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
83-99
96-105
Average (%)
103
91
99
1:4
Range (%)
92-106
97-106
94-103
Average (%)
99
102
99
1:8
Range (%)
85-96
97-106
85-95
Average (%)
91
102
91
1:16
Range (%)
87-100
91-104
88-97
Average (%)
92
99
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
82-96
90
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
92-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.42
6.02
22.29
2.05
6.16
22.81
Standard deviation
0.12
0.29
1.15
0.11
0.29
1.13
C V (%)
4.96
4.82
5.16
5.37
4.71
4.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MAU concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MAU in samples. No significant cross-reactivity or interference between Human MAU and analogues was observed.
