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Human Matrix Metalloproteinase 8 (MMP8) ELISA Kit

SKU:
HUDL01886
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P22894
Sensitivity:
19pg/mL
Range:
39.062-2500pg/mL
ELISA Type:
Sandwich
Synonyms:
CLG2,HNC,PMNL-CL,Collagenase 2,Neutrophil Collagenase,PMNL collagenase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Human Matrix Metalloproteinase 8 (MMP8) ELISA Kit

The Human Matrix Metalloproteinase-8 (MMP8) ELISA Kit is a highly reliable and accurate tool for the quantification of MMP8 levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, providing consistent and precise results for various research applications.MMP8, also known as collagenase-2, is a key enzyme involved in the breakdown of extracellular matrix components, playing a critical role in tissue remodeling and inflammation.

Elevated levels of MMP8 have been associated with various pathological conditions such as arthritis, periodontal diseases, and cancer, highlighting its importance as a biomarker for disease progression and therapeutic development.With its advanced technology and proven performance, the Human MMP8 ELISA Kit is an indispensable tool for researchers studying the role of MMP8 in health and disease, offering valuable insights and potential targets for novel treatments.

Product Name:Human Matrix Metalloproteinase 8 (MMP8) ELISA Kit
Product Code:HUDL01886
Size:96 Assays
Target:MMP8
Synonyms:CLG2, HNC, PMNL-CL, Collagenase 2, Neutrophil Collagenase, PMNL collagenase
Detection Method:Sandwich
Range:39.062-2,500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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