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Human Matrix Metalloproteinase 7 (MMP7) ELISA Kit

SKU:
HUDL01885
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P09237
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MPSL1,MAT,PUMP-1,Matrilysin Uterine,Matrilysin,Matrin,Pump-1 Protease,Uterine Metalloproteinase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Matrix Metalloproteinase 7 (MMP7) ELISA Kit

The Human Matrix Metalloproteinase-7 (MMP7) ELISA Kit is specifically designed for the precise quantification of MMP7 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Matrix metalloproteinase-7 is a key enzyme involved in tissue remodeling and repair, playing a critical role in various physiological and pathological processes. Elevated levels of MMP7 have been linked to conditions such as cancer, inflammatory diseases, and tissue fibrosis, making it a valuable biomarker for studying disease progression and identifying therapeutic targets.

With its advanced technology and reliable performance, the Human MMP7 ELISA Kit is a valuable tool for researchers in the fields of molecular biology, oncology, and immunology. Get accurate and reliable results with this high-quality kit, providing valuable insights into the role of MMP7 in health and disease.

Product Name:Human Matrix Metalloproteinase 7 (MMP7) ELISA Kit
Product Code:HUDL01885
Size:96 Assays
Target:MMP7
Synonyms:MPSL1, MAT, PUMP-1, Matrilysin Uterine, Matrilysin, Matrin, Pump-1 Protease, Uterine Metalloproteinase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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