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Human Matrix Metalloproteinase 3 (MMP3) ELISA Kit

SKU:
HUDL01884
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08254
Sensitivity:
0.068ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SL1,STMY,STMY1,STR1,Progelatinase,Stromelysin 1,Transin-1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Human Matrix Metalloproteinase 3 (MMP3) ELISA Kit

The Human Matrix Metalloproteinase-3 (MMP3) ELISA Kit is a reliable tool for the accurate quantification of MMP3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.MMP3 is a key enzyme involved in extracellular matrix degradation and tissue remodeling. Its dysregulation has been implicated in various pathological conditions such as arthritis, cancer, and cardiovascular diseases.

Therefore, measuring MMP3 levels can provide valuable insights into disease progression and aid in the development of targeted therapeutic approaches.With its user-friendly protocol and robust performance, the Human MMP3 ELISA Kit is an essential tool for researchers investigating the role of MMP3 in health and disease. Get reliable and accurate results with this high-quality ELISA kit from Assay Genie.

Product Name:Human Matrix Metalloproteinase 3 (MMP3) ELISA Kit
Product Code:HUDL01884
Size:96 Assays
Target:MMP3
Synonyms:SL1, STMY, STMY1, STR1, Progelatinase, Stromelysin 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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