The Human MARCKS-related protein (MARCKSL1) ELISA Kit is specifically designed for the precise quantification of MARCKSL1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for various research purposes.MARCKSL1 is a key protein that belongs to the myristoylated alanine-rich C kinase substrate (MARCKS) family, playing a vital role in cellular processes such as membrane trafficking, cytoskeleton organization, and signal transduction.
Dysregulation of MARCKSL1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions, highlighting its significance as a potential biomarker and therapeutic target.With its advanced technology and reliable performance, the Human MARCKSL1 ELISA Kit provides researchers with a valuable tool for studying the role of MARCKSL1 in health and disease, ultimately aiding in the development of novel diagnostic and therapeutic interventions.
Product Name:
Human MARCKS Related Protein (MARCKSL1) ELISA Kit
Product Code:
HUDL01797
Size:
96 Assays
Target:
MARCKSL1
Synonyms:
MLP1, MRP, MLP, F52, MACMARCKS, MARCKS-like protein 1, Macrophage myristoylated alanine-rich C kinase substrate
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
