Human MAPK3 Recombinant Protein (RPPB2578)
- SKU:
- RPPB2578
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P27361
- Research Area:
- Enzymes
Description
| Product Name: | Human MAPK3 Recombinant Protein |
| Product Code: | RPPB2578 |
| Size: | 25µg |
| Species: | Human |
| Target: | MAPK3 |
| Synonyms: | ERK1, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAP kinase3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MAPK3 0.5mg/ml protein solution contains 20mM Tris-HCl buffer pH-8, 1mM DTT, 0.1M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV IGIRDILRAS TLEAMRDVYI VQDLMETDLY KLLKSQQLSN DHICYFLYQI LRGLKYIHSA NVLHRDLKPS NLLINTTCDL KICDFGLARI ADPEHDHTGF LTEYVATRWY RAPEIMLNSK GYTKSIDIWS VGCILAEMLS NRPIFPGKHY LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD SKALDLLDRM LTFNPNKRIT VEEALAHPYL EQYYDPTDEP VAEEPFTFAM ELDDLPKERL KELIFQETAR FQPGVLEAP |
MAPK3 is a part of the MAP kinase family which is recognized as extracellular signal-regulated kinases (ERKs), that function in a signaling cascade that controls various cellular procedures such as proliferation, differentiation, and cell cycle progression in reaction to a variety of extracellular signals. MAPK3 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
MAPK3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 399 amino acids (1-379 a.a.) and having a molecular mass of 45.2 kDa. The MAPK3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. |
| UniProt Protein Details: | Protein type:EC 2.7.11.24; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: microtubule cytoskeleton; nucleoplasm; Golgi apparatus; focal adhesion; cytoskeleton; mitochondrion; late endosome; early endosome; nuclear envelope; caveola; pseudopodium; nucleus; cytosol Molecular Function:MAP kinase activity; protein binding; phosphotyrosine binding; ATP binding; phosphatase binding Biological Process: axon guidance; DNA damage induced protein phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; positive regulation of histone phosphorylation; viral reproduction; apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; sensory perception of pain; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; BMP signaling pathway; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; small GTPase mediated signal transduction; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; transcription from RNA polymerase I promoter; cell cycle; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of stress-activated MAPK cascade; organ morphogenesis; cartilage development; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; gene expression; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; toll-like receptor 9 signaling pathway; transcription initiation from RNA polymerase I promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; phosphorylation; regulation of cytoskeleton organization and biogenesis |
| NCBI Summary: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P27361 |
| NCBI GenInfo Identifier: | 232066 |
| NCBI Gene ID: | 5595 |
| NCBI Accession: | P27361.4 |
| UniProt Secondary Accession: | P27361,Q8NHX1, A8CZ58, B0LPG3, |
| UniProt Related Accession: | P27361 |
| Molecular Weight: | 379 |
| NCBI Full Name: | Mitogen-activated protein kinase 3 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase 3 |
| NCBI Official Symbol: | MAPK3�� |
| NCBI Official Synonym Symbols: | ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK�� |
| NCBI Protein Information: | mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase |
| UniProt Protein Name: | Mitogen-activated protein kinase 3 |
| UniProt Synonym Protein Names: | ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1 |
| UniProt Gene Name: | MAPK3�� |
| UniProt Entry Name: | MK03_HUMAN |
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