Human MAP-2 (Microtubule Associated Protein 2) ELISA Kit
The Human MAP-2 (Microtubule Associated Protein 2) ELISA Kit is an advanced assay developed for the quantitative measurement of MAP-2 levels in human biological samples.
MAP-2 is a key neuronal protein involved in stabilizing microtubules within the cytoskeleton of neurons and plays a crucial role in neuronal development, plasticity, and function. Alterations in MAP-2 expression levels are linked to various neurodevelopmental and neurodegenerative disorders, making it a valuable biomarker for assessing neuronal health and integrity. This ELISA kit enables researchers to precisely quantify MAP-2 levels, providing essential information on neuronal health, synaptic connectivity, and neuroplasticity. By accurately measuring MAP-2 concentrations, this assay aids in understanding neuronal pathophysiology and can serve as a valuable tool in neuroscientific research, drug discovery, and biomarker studies related to brain disorders. The Human MAP-2 ELISA Kit ensures exceptional sensitivity and specificity, delivering reliable and reproducible results. Manufactured under stringent quality control protocols, this kit offers robust performance and ease of use, making it an ideal choice for neurobiology and neuroscience research applications.
Product Name:
Human MAP-2 (Microtubule Associated Protein 2) ELISA Kit
SKU:
AEES00696
Target:
Human MAP-2 (Microtubule Associated Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MAP-2. You can calculate the concentration of Human MAP-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
88-104
90-104
Average (%)
97
96
97
1:4
Range (%)
86-100
80-93
88-103
Average (%)
92
86
95
1:8
Range (%)
89-104
86-96
85-98
Average (%)
97
91
91
1:16
Range (%)
89-101
79-90
82-95
Average (%)
94
86
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
2.09
8.34
1.0
2.26
8.79
Standard deviation
0.07
0.1
0.43
0.06
0.13
0.45
C V (%)
6.67
4.78
5.16
6.0
5.75
5.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MAP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MAP-2 in samples. No significant cross-reactivity or interference between Human MAP-2 and analogues was observed.
