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Human Mannose Receptor C Type 2 (MRC2) ELISA Kit

SKU:
HUDL01903
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UBG0
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD280,ENDO180,CLEC13E,UPARAP,C-type lectin domain family 13 member E,Endocytic receptor 180,Macrophage mannose receptor 2,Urokinase receptor-associated protein
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Mannose Receptor C Type 2 (MRC2) ELISA Kit

The Human Mannose Receptor C-Type 2 (MRC2) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of MRC2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent tool for a wide variety of research applications.MRC2 is a key receptor involved in various cellular processes, including the uptake of mannose-containing glycoproteins and the regulation of extracellular matrix turnover.

Dysregulation of MRC2 has been implicated in diseases such as cancer, fibrosis, and inflammatory disorders, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.Overall, the Human Mannose Receptor C-Type 2 (MRC2) ELISA Kit offers researchers a valuable tool for investigating the role of MRC2 in health and disease, providing crucial insights into potential therapeutic interventions.

Product Name:Human Mannose Receptor C Type 2 (MRC2) ELISA Kit
Product Code:HUDL01903
Size:96 Assays
Target:MRC2
Synonyms:CD280, ENDO180, CLEC13E, UPARAP, C-type lectin domain family 13 member E, Endocytic receptor 180, Macrophage mannose receptor 2, Urokinase receptor-associated protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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