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Human Mannose Binding Lectin (MBL) ELISA Kit

SKU:
HUDL01806
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11226
Sensitivity:
0.068ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MBL2,COLEC1,HSMBPC,MBP1,MBP,Collectin-1,Mannose-binding protein C,Mannan Binding Protein,Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Mannose Binding Lectin (MBL) ELISA Kit

The Human Mannose Binding Lectin (MBL) ELISA Kit is a specialized assay designed for the precise measurement of mannose-binding lectin levels in human samples, including serum, plasma, and cell culture supernatants. This ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Mannose-binding lectin is a key component of the innate immune system, playing a crucial role in pathogen recognition and clearance.

Dysregulation of MBL levels has been linked to various infectious diseases and autoimmune disorders, highlighting its importance as a biomarker for disease research and therapeutic development.With its high-quality components and reliable performance, the Human Mannose Binding Lectin (MBL) ELISA Kit is a valuable tool for scientists and researchers seeking to deepen their understanding of the immune response and explore potential targets for intervention.

Product Name:Human Mannose Binding Lectin (MBL) ELISA Kit
Product Code:HUDL01806
Size:96 Assays
Target:MBL
Synonyms:MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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