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Human Mannose Associated Serine Protease 2 (MASP2) ELISA Kit

SKU:
HUDL01799
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O00187
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Mannose-binding protein-associated serine protease 2,mannan-Binding Lectin Serine Peptidase 2,MBL-associated serine protease 2
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human Mannose Associated Serine Protease 2 (MASP2) ELISA Kit

The Human Mannose-associated Serine Protease 2 (MASP2) ELISA Kit is specifically designed for the accurate quantification of MASP2 levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MASP2 is a critical component of the lectin pathway of the complement system, playing a key role in innate immunity and inflammatory responses.

Dysregulation of MASP2 has been implicated in various autoimmune diseases, infections, and inflammatory conditions, making it a valuable biomarker for understanding and developing treatments for these disorders.With its advanced technology and reliable performance, the Human MASP2 ELISA Kit is an essential tool for researchers studying immunology, infectious diseases, and autoimmune disorders. Trust in this kit to provide accurate and valuable data for your research needs.

Product Name:Human Mannose Associated Serine Protease 2 (MASP2) ELISA Kit
Product Code:HUDL01799
Size:96 Assays
Target:MASP2
Synonyms:Mannose-binding protein-associated serine protease 2, mannan-Binding Lectin Serine Peptidase 2, MBL-associated serine protease 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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