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Human Malate Dehydrogenase 1 (MDH1) ELISA Kit

SKU:
HUDL01822
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P40925
Sensitivity:
27pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
MDHA,Malate Dehydrogenase 1,NAD(Soluble),Cytosolic malate dehydrogenase,Diiodophenylpyruvate reductase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Malate Dehydrogenase 1 (MDH1) ELISA Kit

The Human Malate Dehydrogenase 1 (MDH1) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of MDH1 levels in human serum, plasma, and cell culture supernatants. MDH1 is a key enzyme involved in the citric acid cycle and plays a crucial role in energy production within cells.This ELISA kit provides accurate and reproducible results, making it an excellent tool for researchers studying metabolic pathways, cellular energy metabolism, and diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.

By measuring MDH1 levels, researchers can gain valuable insights into cellular metabolism and potential therapeutic targets for various conditions. Overall, the Human MDH1 ELISA Kit is a valuable research tool for studying the role of MDH1 in health and disease, offering reliable data for a wide range of applications in the biomedical field.

Product Name:Human Malate Dehydrogenase 1 (MDH1) ELISA Kit
Product Code:HUDL01822
Size:96 Assays
Target:MDH1
Synonyms:MDHA, Malate Dehydrogenase 1,NAD(Soluble), Cytosolic malate dehydrogenase, Diiodophenylpyruvate reductase
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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