Human Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) ELISA Kit
The Human Major Histocompatibility Complex Class II DR Beta 1 (MHCDRB1) ELISA Kit is a reliable tool for the sensitive detection of MHCDRB1 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for a wide range of research applications.MHCDRB1 is a key component of the immune system, playing a crucial role in immune responses and regulation. It is involved in presenting antigens to T cells, ultimately influencing immune reactions and immune system function.
Research on MHCDRB1 can provide valuable insights into autoimmune disorders, infectious diseases, and transplant rejection, making this ELISA kit essential for researchers studying immunology and related fields.Overall, the Human Major Histocompatibility Complex Class II DR Beta 1 (MHCDRB1) ELISA Kit offers a comprehensive solution for the detection and quantification of MHCDRB1 levels, enabling researchers to advance their understanding of immune responses and related conditions.
Product Name:
Human Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) ELISA Kit
Product Code:
HUDL01849
Size:
96 Assays
Target:
MHCDRb1
Synonyms:
HLA-DRB1, HLA-DR1B, HLADRb1, HLA Class II Histocompatibility Antigen,DRB1-9 Beta Chain, HLA class II histocompatibility antigen, DRB1-15 beta chain
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-100
90
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
