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Human Macrophage Scavenger Receptor 1 (MSR1) ELISA Kit

SKU:
HUDL01911
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P21757
Sensitivity:
0.133ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD204,MSR1,SCARA1,SR-A,PhSR1,PhSR2,Macrophage acetylated LDL receptor I and II,Scavenger receptor class A member 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Macrophage Scavenger Receptor 1 (MSR1) ELISA Kit

The Human Macrophage Scavenger Receptor 1 (MSR1) ELISA Kit is specially designed for the accurate detection of MSR1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.MSR1 is a key receptor involved in the recognition and clearance of modified lipoproteins, playing a crucial role in the regulation of lipid metabolism and the immune response. Dysregulation of MSR1 has been linked to a variety of diseases, including atherosclerosis, Alzheimer's disease, and diabetes, making it a valuable biomarker for studying these conditions and exploring potential treatment options.

With its user-friendly format and reliable performance, the Human MSR1 ELISA Kit is an essential tool for researchers seeking to investigate the role of MSR1 in disease pathogenesis and therapeutic interventions. Get accurate and insightful results with this cutting-edge ELISA kit from Assay Genie.

Product Name:Human Macrophage Scavenger Receptor 1 (MSR1) ELISA Kit
Product Code:HUDL01911
Size:96 Assays
Target:MSR1
Synonyms:CD204, MSR1, SCARA1, SR-A, PhSR1, PhSR2, Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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