Human M CSF Recombinant Protein (RPPB0749)
- SKU:
- RPPB0749
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P09603
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Human M CSF Recombinant Protein |
| Product Code: | RPPB0749 |
| Size: | 10µg |
| Species: | Human |
| Target: | M CSF |
| Synonyms: | CSF-1, Lanimostim, MCSF, MGC31930, M-CSF, Macrophage colony-stimulating factor 1, CSF1. |
| Source: | Baculovirus infected Silkworm |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The lyophilized protein (1mg/ml) was lyophilized with 20mM phosphate buffer, 1% HSA and 3% manntiol. |
| Solubility: | It is recommended to reconstitute the lyophilized M-CSF in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQ |
| Biological Activity: | The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 3ng/ml, corresponding to a specific activity of less than 333,333.33units/mg. |
Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Macrophage Colony Stimulating Factor Human Recombinant produced in Baculovirus is a disulfide linked homodimer, glycosylated, polypeptide chain containing 2 x 149 amino acids and having a total molecular mass of 42 kDa.MCSF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine; Membrane protein, integral Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: extracellular space; perinuclear region of cytoplasm Molecular Function:cytokine activity; growth factor activity; macrophage colony stimulating factor receptor binding; protein binding; protein homodimerization activity Biological Process: macrophage differentiation; osteoclast differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of cellular protein metabolic process; positive regulation of macrophage differentiation; positive regulation of monocyte differentiation; positive regulation of mononuclear cell proliferation; positive regulation of osteoclast differentiation; positive regulation of protein kinase activity; reproductive developmental process; transmembrane receptor protein tyrosine kinase signaling pathway |
| NCBI Summary: | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P09603 |
| NCBI GenInfo Identifier: | 311033367 |
| NCBI Gene ID: | 1435 |
| NCBI Accession: | P09603.2 |
| UniProt Secondary Accession: | P09603,Q13130, Q14086, Q14806, Q5VVF3, Q5VVF4, Q9UQR8 A8K6J5, |
| UniProt Related Accession: | P09603 |
| Molecular Weight: | 29,215 Da |
| NCBI Full Name: | Macrophage colony-stimulating factor 1 |
| NCBI Synonym Full Names: | colony stimulating factor 1 |
| NCBI Official Symbol: | CSF1�� |
| NCBI Official Synonym Symbols: | MCSF; CSF-1�� |
| NCBI Protein Information: | macrophage colony-stimulating factor 1 |
| UniProt Protein Name: | Macrophage colony-stimulating factor 1 |
| UniProt Synonym Protein Names: | Lanimostim |
| Protein Family: | Macrophage colony-stimulating factor |
| UniProt Gene Name: | CSF1�� |
| UniProt Entry Name: | CSF1_HUMAN |
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