The Human LYZ (Lysozyme) ELISA Kit is specifically designed for the sensitive and accurate detection of lysozyme levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Lysozyme is an important enzyme that plays a key role in the immune system by breaking down bacterial cell walls. It is also involved in various physiological processes, such as wound healing and digestion.
Dysregulation of lysozyme levels has been associated with inflammatory diseases, infections, and certain cancers, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.Overall, the Human LYZ (Lysozyme) ELISA Kit provides researchers with a powerful tool for exploring the role of lysozyme in health and disease, offering valuable insights into potential diagnostic and treatment options.
Product Name:
Human LZM (Lysozyme) ELISA Kit
SKU:
HUES02821
Target:
Human LZM (Lysozyme)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LZM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LZM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LZM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LZM. You can calculate the concentration of Human LZM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
87-100
93-109
Average (%)
104
93
101
1:4
Range (%)
93-105
82-94
87-100
Average (%)
99
87
94
1:8
Range (%)
86-101
85-95
84-96
Average (%)
92
90
90
1:16
Range (%)
86-99
85-100
87-101
Average (%)
92
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.18
12.47
33.07
4.01
12.05
31.34
Standard deviation
0.26
0.53
1.16
0.22
0.72
1.49
C V (%)
6.22
4.25
3.51
5.49
5.98
4.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LZM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LZM in samples. No significant cross-reactivity or interference between Human LZM and analogues was observed.
