The Human Lysyl-tRNA Synthetase (KARS) ELISA Kit is a powerful tool for measuring levels of lysyl-tRNA synthetase in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it suitable for a wide range of research applications.Lysyl-tRNA synthetase is an essential enzyme that plays a critical role in protein synthesis by attaching the amino acid lysine to its corresponding tRNA molecule. Dysregulation of this enzyme has been implicated in various diseases, including cancer and metabolic disorders, underscoring its importance as a potential biomarker for disease pathology and therapeutic development.
Researchers seeking to explore the roles of lysyl-tRNA synthetase in health and disease can rely on the Human Lysyl-tRNA Synthetase (KARS) ELISA Kit for precise and reliable quantification of this key enzyme. Its ease of use and accurate results make it an invaluable asset in unraveling the intricate mechanisms underlying lysyl-tRNA synthetase function.
Product Name:
Human Lysyl tRNA Synthetase (KARS) ELISA Kit
Product Code:
HUDL01595
Size:
96 Assays
Target:
KARS
Synonyms:
KARS2, LysRS, Lysine tRNA Ligase
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
