Human Lysyl Oxidase Like Protein 1 (LOXL1) ELISA Kit
The Human Lysyl Oxidase-Like Protein 1 (LOXL1) ELISA Kit from Assay Genie is specifically designed to accurately detect levels of LOXL1 in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it a valuable tool for a wide range of research applications.LOXL1 is a key enzyme involved in the cross-linking of collagen and elastin, essential for the structural integrity of connective tissues. Dysregulation of LOXL1 has been linked to various diseases, including fibrosis, cancer, and cardiovascular disorders, highlighting its importance as a potential therapeutic target and biomarker for disease progression.
By using the Human LOXL1 ELISA Kit, researchers can gain valuable insights into the role of LOXL1 in disease pathogenesis and explore potential treatment strategies. Trust Assay Genie for accurate and reliable measurements of LOXL1 levels in biological samples, advancing your research efforts in the field of connective tissue disorders and beyond.
Product Name:
Human Lysyl Oxidase Like Protein 1 (LOXL1) ELISA Kit
Product Code:
HUDL01737
Size:
96 Assays
Target:
LOXL1
Synonyms:
LOL, LOXL, Lysyl oxidase homolog 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
