The Human LY96 (Lymphocyte Antigen 96) ELISA Kit is specifically designed for the precise and accurate measurement of LY96 levels in human samples including serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.LY96, also known as MD-2, is a key component of the Toll-like receptor 4 (TLR4) signaling pathway, which plays a critical role in the immune response to bacterial infections and inflammation.
Abnormalities in LY96 expression have been linked to various inflammatory diseases, making it a valuable biomarker for studying these conditions and potentially developing new therapeutic strategies.With the Human LY96 ELISA Kit, researchers can accurately measure LY96 levels in biological samples, providing insights into the role of this protein in inflammatory processes and immune responses. This kit is a valuable tool for advancing research in immunology, infectious diseases, and inflammatory disorders.
Product Name:
Human Lymphocyte Antigen 96 (LY96) ELISA Kit
Product Code:
HUDL01771
Size:
96 Assays
Target:
LY96
Synonyms:
MD-2, ESOP1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
