TNFCR,TNFR-RP,TNFR-III,TNFR2-RP,TNFRSF3,Tumor Necrosis Factor Superfamily Member 3,Tumor necrosis factor C receptor,Tumor necrosis factor receptor type III
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Human Lymphotoxin Beta Receptor (LTBR) ELISA Kit is specifically designed for the precise detection of LTBR levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for various research applications.LTBR is a receptor protein that plays a critical role in immune response regulation and inflammation. It is involved in signaling pathways that control cell survival, apoptosis, and immune cell activation. Dysregulation of LTBR has been linked to various autoimmune diseases, inflammatory disorders, and cancer, highlighting its importance as a biomarker in understanding disease processes and potential therapeutic interventions.
By using the Human LTBR ELISA Kit, researchers can gain valuable insights into the role of LTBR in various diseases and explore its potential as a target for novel treatment strategies. This kit offers a reliable tool for studying LTBR levels and dynamics, providing a deeper understanding of its biological functions and clinical implications.
Product Name:
Human Lymphotoxin Beta Receptor (LTbR) ELISA Kit
Product Code:
HUDL01767
Size:
96 Assays
Target:
LTbR
Synonyms:
TNFCR, TNFR-RP, TNFR-III, TNFR2-RP, TNFRSF3, Tumor Necrosis Factor Superfamily Member 3, Tumor necrosis factor C receptor, Tumor necrosis factor receptor type III
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
