Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) CLIA Kit
The Human LRG1 (Leucine-Rich Alpha-2-Glycoprotein 1) CLIA Kit is a cutting-edge assay designed for the accurate quantification of LRG1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results, making it a valuable tool for a wide range of research studies.LRG1 is a key protein involved in various physiological processes, including immune response, inflammatory signaling, and tumor development.
Abnormal levels of LRG1 have been associated with numerous health conditions, including autoimmune diseases, cancer, and cardiovascular disorders. Therefore, the Human LRG1 CLIA Kit is an indispensable resource for researchers looking to investigate the role of LRG1 in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) CLIA Kit
SKU:
HUES00739
Target:
Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LRG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LRG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LRG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LRG1. You can calculate the concentration of Human LRG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
88-99
85-96
Average (%)
97
93
91
1:4
Range (%)
90-102
96-111
88-102
Average (%)
97
102
94
1:8
Range (%)
86-98
93-107
96-108
Average (%)
93
99
102
1:16
Range (%)
88-100
88-101
87-101
Average (%)
94
93
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
98-115
106
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.11
13.82
40.86
4.62
13.45
38.37
Standard deviation
0.54
1.64
4.65
0.55
1.24
3.26
C V (%)
10.57
11.87
11.38
11.9
9.22
8.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LRG1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LRG1 in samples. No significant cross-reactivity or interference between Human LRG1 and analogues was observed.
