Human LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit
The Human LP-PLA2 (Phospholipase A2) ELISA Kit is specifically designed for the accurate measurement of LP-PLA2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and consistent results, which makes it an excellent choice for various research applications.LP-PLA2, also known as lipoprotein-associated phospholipase A2, is an enzyme that plays a crucial role in the inflammation and development of atherosclerosis. Elevated levels of LP-PLA2 have been linked to an increased risk of cardiovascular events, making it a valuable biomarker for assessing cardiovascular disease risk and monitoring treatment effectiveness.
By utilizing the Human LP-PLA2 ELISA Kit, researchers can gain valuable insights into the role of LP-PLA2 in various health conditions, including heart disease and stroke, and potentially identify new therapeutic targets for intervention. Overall, this kit provides a reliable tool for advancing our understanding of LP-PLA2 biology and its implications for human health.
Product Name:
Human LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit
SKU:
HUES03168
Target:
Human LpPLA2 (Phospholipase A2, Lipoprotein Associated)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LpPLA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LpPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LpPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LpPLA2. You can calculate the concentration of Human LpPLA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
86-101
88-102
Average (%)
96
93
95
1:4
Range (%)
90-105
81-91
83-97
Average (%)
98
86
88
1:8
Range (%)
88-103
85-96
82-97
Average (%)
95
90
89
1:16
Range (%)
86-100
87-101
85-98
Average (%)
92
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
2.92
9.75
1.07
2.88
9.79
Standard deviation
0.06
0.16
0.5
0.06
0.12
0.44
C V (%)
5.77
5.48
5.13
5.61
4.17
4.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LpPLA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LpPLA2 in samples. No significant cross-reactivity or interference between Human LpPLA2 and analogues was observed.
