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Human Low Density Lipoprotein Receptor Related Protein 6 (LRP6) ELISA Kit

SKU:
HUDL01757
Product Type:
ELISA Kit
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
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Description

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Human Low Density Lipoprotein Receptor Related Protein 6 (LRP6) ELISA Kit

The Human Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) ELISA Kit is a reliable and accurate tool for the detection of LRP6 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it ideal for a variety of research applications.LRP6 is a key protein involved in the Wnt signaling pathway, playing a crucial role in cell proliferation, differentiation, and development.

Dysregulation of LRP6 has been linked to various diseases, including cancer and metabolic disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human LRP6 ELISA Kit offers researchers a powerful tool for investigating the role of LRP6 in disease pathology and developing novel therapeutic strategies.

Product Name:Human Low Density Lipoprotein Receptor Related Protein 6 (LRP6) ELISA Kit
Product Code:HUDL01757
Size:96 Assays
Target:LRP6
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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