null

Human Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit

SKU:
HUDL01753
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q07954
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD91,LRP,A2MR,APOER,APR,TGFBR5,Alpha-2-Macroglobulin Receptor,Prolow-density lipoprotein receptor-related protein 1,Apolipoprotein E receptor
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit

The Human Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) ELISA Kit is specifically designed for the accurate measurement of LRP1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.LRP1 is a key protein involved in lipid metabolism and cellular signaling, playing a critical role in the regulation of cholesterol levels and clearance of lipoproteins from the bloodstream.

Dysregulation of LRP1 has been linked to various cardiovascular and neurodegenerative diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human LRP1 ELISA Kit provides researchers with a powerful tool for studying the role of LRP1 in health and disease, offering a reliable and convenient method for quantifying LRP1 levels in biological samples.

Product Name:Human Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit
Product Code:HUDL01753
Size:96 Assays
Target:LRP1
Synonyms:CD91, LRP, A2MR, APOER, APR, TGFBR5, Alpha-2-Macroglobulin Receptor, Prolow-density lipoprotein receptor-related protein 1, Apolipoprotein E receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products