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Human Liver Receptor Homolog 1 (LRH1) ELISA Kit

SKU:
HUDL01752
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O00482
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NR5A2,B1F,B1F2,CPF,FTF,FTZ-F1,LRH-1,hB1F,hB1F-2,Hepatocytic transcription factor,Alpha-1-fetoprotein transcription factor,Nuclear Receptor Subfamily 5,Group A,Member 2
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Liver Receptor Homolog 1 (LRH1) ELISA Kit

The Human Liver Receptor Homolog 1 (LRH1) ELISA Kit is specifically designed for the accurate measurement of LRH1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.LRH1 is a key transcription factor involved in the regulation of genes related to lipid metabolism, bile acid synthesis, and glucose homeostasis. Dysregulation of LRH1 has been linked to metabolic disorders, liver diseases, and certain cancers, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.

By utilizing the Human Liver Receptor Homolog 1 ELISA Kit, researchers can gain valuable insights into the role of LRH1 in various physiological and pathological processes, ultimately advancing our understanding of these complex diseases and facilitating the development of novel treatment strategies.

Product Name:Human Liver Receptor Homolog 1 (LRH1) ELISA Kit
Product Code:HUDL01752
Size:96 Assays
Target:LRH1
Synonyms:NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, LRH-1, hB1F, hB1F-2, Hepatocytic transcription factor, Alpha-1-fetoprotein transcription factor, Nuclear Receptor Subfamily 5,Group A,Member 2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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