The Human LIFE (Leukemia Inhibitory Factor) CLIA Kit is specifically designed for the precise measurement of leukemia inhibitory factor levels in human serum, plasma, and cell culture supernatants. This easy-to-use kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Leukemia inhibitory factor is a key cytokine that plays a critical role in regulating hematopoiesis, immune response, and inflammation.
It is implicated in various diseases including leukemia, inflammatory disorders, and reproductive issues, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human LIFE CLIA Kit provides researchers with a powerful tool to investigate the role of leukemia inhibitory factor in health and disease, advancing our understanding of these complex pathways and supporting the development of innovative treatments.
Product Name:
Human LIF (Leukemia Inhibitory Factor) CLIA Kit
SKU:
HUES00090
Target:
Human LIF (Leukemia Inhibitory Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LIF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LIF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LIF. You can calculate the concentration of Human LIF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-117
94-112
102-114
Average (%)
107
102
108
1:4
Range (%)
98-112
98-111
89-104
Average (%)
105
105
95
1:8
Range (%)
86-96
86-100
100-113
Average (%)
91
93
107
1:16
Range (%)
92-107
101-116
89-101
Average (%)
98
107
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.17
85.53
303.63
37.36
79.49
320.35
Standard deviation
4.53
9.03
25.72
4.85
5.91
27.97
C V (%)
12.19
10.56
8.47
12.98
7.43
8.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LIF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LIF in samples. No significant cross-reactivity or interference between Human LIF and analogues was observed.
