The Human LGALS3BP (Lectin galactoside-binding soluble 3-binding protein) ELISA Kit is specially designed for accurate detection of LGALS3BP levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.LGALS3BP is a vital protein that plays a key role in cell adhesion, migration, and invasion processes. It is closely associated with cancer progression, immune response modulation, and inflammatory pathways, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Human LGALS3BP ELISA Kit offers researchers a powerful tool to investigate the role of LGALS3BP in various physiological and pathological processes, providing valuable insights into potential diagnostic and therapeutic strategies.
Human LGALS3BP (Lectin Galactoside Binding, Soluble 3 Binding Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LGALS3BP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LGALS3BP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LGALS3BP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LGALS3BP. You can calculate the concentration of Human LGALS3BP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
86-98
87-99
Average (%)
106
92
93
1:4
Range (%)
86-102
82-95
81-96
Average (%)
93
89
88
1:8
Range (%)
91-107
88-102
85-99
Average (%)
98
93
91
1:16
Range (%)
89-105
87-98
85-98
Average (%)
96
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
102
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.24
7.47
27.6
4.61
7.13
26.58
Standard deviation
0.23
0.3
1.35
0.29
0.42
1.2
C V (%)
5.42
4.02
4.89
6.29
5.89
4.51
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LGALS3BP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LGALS3BP in samples. No significant cross-reactivity or interference between Human LGALS3BP and analogues was observed.
