The Human LGALS3BP (Lectin Galactoside-Binding Soluble 3 Binding Protein) CLIA Kit is specifically designed for the quantitative measurement of LGALS3BP levels in human serum, plasma, and cell culture supernatants. This kit ensures high sensitivity and specificity, allowing for accurate and reliable results for a variety of research applications.LGALS3BP is a critical protein that plays a role in various biological processes, including cell adhesion, migration, and angiogenesis.
It is involved in promoting tumor growth, metastasis, and immune response modulation, making it a valuable biomarker for studying cancer, inflammation, and other related conditions.With the Human LGALS3BP CLIA Kit, researchers can precisely measure LGALS3BP levels in biological samples, providing valuable insight into disease mechanisms and potential therapeutic targets. Get reliable and reproducible results with this advanced kit for in-depth research studies.
Human LGALS3BP (Lectin Galactoside Binding, Soluble 3 Binding Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LGALS3BP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LGALS3BP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LGALS3BP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LGALS3BP. You can calculate the concentration of Human LGALS3BP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
86-98
99-113
Average (%)
94
91
106
1:4
Range (%)
92-107
100-117
89-102
Average (%)
98
108
96
1:8
Range (%)
89-103
86-97
86-97
Average (%)
95
92
91
1:16
Range (%)
93-108
96-112
86-96
Average (%)
101
103
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
2.89
9.45
0.99
2.97
9.8
Standard deviation
0.09
0.25
0.88
0.13
0.25
0.72
C V (%)
9.47
8.65
9.31
13.13
8.42
7.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LGALS3BP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LGALS3BP in samples. No significant cross-reactivity or interference between Human LGALS3BP and analogues was observed.
